In Situ Transcriptome Accessibility Sequencing (INSTA-seq)
bioRxiv 130d 4 tweets
Subcellular RNA localization regulates spatially polarized cellular processes, but unbiased investigation of its control in vivo remains challenging. Current hybridization-based methods cannot differentiate small regulatory variants, while in situ...


Joshua Levin 128d ago
In Situ Transcriptome Accessibility Sequencing (INSTA-seq) cool method!
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Vijay Ramani 130d ago
This new in situ transcriptomic approach seems incredibly cool/elegant; could address a lot of really interesting biology, including subcellular localization of protein-RNA interactions / characterization of RNA regulons: congrats & authors!!
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Jay Shendure 129d ago
Incredibly cool and creative paper from at . Opens the door to in situ full-length (!) mRNA sequencing as well as high-res in situ mapping of RNA protein interactions.
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Keith Robison 129d ago
Now here’s a different take on sequencing: by ligation, extending the primer in *both* directions each cycle
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