In Situ Transcriptome Accessibility Sequencing (INSTA-seq)

bioRxiv 374d 4 tweets

Subcellular RNA localization regulates spatially polarized cellular processes, but unbiased investigation of its control in vivo remains challenging. Current hybridization-based methods cannot differentiate small regulatory variants, while in situ...

Joshua Levin 372d ago

In Situ Transcriptome Accessibility Sequencing (INSTA-seq) biorxiv.org/content/10.110… cool method!

Vijay Ramani 374d ago

This new in situ transcriptomic approach seems incredibly cool/elegant; could address a lot of really interesting biology, including subcellular localization of protein-RNA interactions / characterization of RNA regulons: biorxiv.org/content/10.110… congrats @jlee8usa & authors!!

Jay Shendure 373d ago

Incredibly cool and creative paper from @jlee8usa at @CSHL. Opens the door to in situ full-length (!) mRNA sequencing as well as high-res in situ mapping of RNA protein interactions.

Keith Robison 373d ago

Now here’s a different take on sequencing: by ligation, extending the primer in *both* directions each cycle