In Situ Transcriptome Accessibility Sequencing (INSTA-seq) cool method!
This new in situ transcriptomic approach seems incredibly cool/elegant; could address a lot of really interesting biology, including subcellular localization of protein-RNA interactions / characterization of RNA regulons: congrats & authors!!
Incredibly cool and creative paper from at . Opens the door to in situ full-length (!) mRNA sequencing as well as high-res in situ mapping of RNA protein interactions.
Now here’s a different take on sequencing: by ligation, extending the primer in *both* directions each cycle