Exploration of Bacterial Bottlenecks and Streptococcus pneumoniae Pathogenesis by CRISPRi-Seq
Liu et al. developed CRISPRi-seq to enable in vivo genome-wide fitness testing of
Streptococcus pneumoniae in one sequencing step. CRISPRi-seq revealed a bottleneck
during murine pneumococcal infection not observed upon influenza virus...
Jan-Willem Veening
81d ago
Extremely happy with our latest paper @cellhostmicrobe in which we develop CRISPRi-seq for Streptococcus pneumoniae and use it to explore infection bottlenecks and genes important for infection in vivo. A short thread 1/13
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CRISPRi-Seq unveils bacterial bottlenecks: @JWVeening &Co develop CRISPRi-seq for in vivo genome-wide fitness testing of #Spneumoniae. ID bottleneck during pneumococcal infection not seen w/ #influenza coinfection enabling identification of essential genes cell.com/cell-host-micr… pic.twitter.com/fSiapPqC4h
