Extremely happy with our latest paper in which we develop CRISPRi-seq for Streptococcus pneumoniae and use it to explore infection bottlenecks and genes important for infection in vivo. A short thread 1/13
CRISPRi-Seq unveils bacterial bottlenecks: &Co develop CRISPRi-seq for in vivo genome-wide fitness testing of #Spneumoniae. ID bottleneck during pneumococcal infection not seen w/ #influenza coinfection enabling identification of essential genes